货号：558621

品牌：BD Pharmingen

规格：1EA

目录价：￥880.00

市场价格：￥554.40

会员价格：￥484.00

金山科研平台，产品价格货期咨询微信：jinshanbio

Interferon regulatory factor 7 (IRF-7) is a transcription factor that regulates anti-viral defenses by controlling the induction of type-I interferon (IFN) responses. IRF-7 expression is induced in lymphoid cells by virus infection, as well as by IFN, lipopolysaccharide, and TNF-a. IRF-7 responses are initiated by Toll-like receptors (TLR) or the cytoplasmic protein retinoic acid inducible gene I (RIG-I). Upon TLR activation, it forms cytoplasmic complexes with MyD88, an adaptor in the TLR signaling pathways. The TLR-dependent and RIG-I-dependent pathways activate kinases, such as IKK-e and TBK1, that phosphorylate IRF-7 and induce movement of IRF-7-containing complexes to the nucleus, where it preferentially activates IFN-a promotersThe K47-671 monoclonal antibody recognizes human IRF-7 phosphorylated at serines 477 and 479 (pS477/pS479). Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7. Phosphorylation of IRF-7 in the transfectants requires virus infection or over-expression of a signaling molecule of the RIG-I pathway, such as TBK1. Phosphorylation of endogenous IRF-7 in untransfected cells has not yet been detected. We confirmed that mAb K47-671 does not cross-react with TBK1 by Western blot analysis using the purified antibody.

Catalog Number

558621

Brand

BD Phosflow™

Name

IRF-7 (pS477/pS479)

Size

50Tests

RegulatoryStatus

RUO

Clone

K47-671

Format

PE

Isotype

IgG₁, κ

W.B. Positive Control

none

Immunogen

Phosphorylated Human IRF-7 Peptide

Strain Host

BALB/c

Reactivity

QC Testing : Human

Applications

Intracellular staining (flow cytometry)

- Routinely Tested