货号：560312

品牌：BD Pharmingen

规格：1EA

目录价：￥4368.00

市场价格：￥2751.84

会员价格：￥2402.40

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Stat (Signal transducer and activators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation. Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes. Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat3 is a 92-kDa protein that is activated as a DNA- binding protein through cytokines, such as IL-6, and growth factors, such as EGF. Stat3 activation occurs via tyrosine phosphorylation at Y705. Tyrosine phosphorylation in response to cytokine stimulation is generally mediated by JAK1. Upon activation, Stat3 dimerizes, translocates to the nucleus and binds DNA response elements, thereby regulating gene expression. It has been reported that Stat3 binds to DNA as a homodimer, but it is also capable of binding as a heterodimer with Stat1. In addition to tyrosine phosphorylation, Stat3 is also phosphorylated at S727 via the MAPK pathway. Stat3 is widely expressed and can bind to the sis-inducible element (SIE) site from the c-fos promoter. This site is similar to the GAS element that is present in IFN-γ induced genes. Thus, phosphorylation of Y705 in Stat3 occurs in response to growth factors and cytokines, and is essential for normal transcription activity.

Catalog Number

560312

Brand

BD Phosflow™

Name

Stat3 (pY705)

Size

50Tests

RegulatoryStatus

RUO

Clone

4/P-STAT3

Format

Pacific Blue™

Isotype

IgG_2a, κ

W.S. No

none

W.B. Positive Control

none

Molecular Weight

none

Immunogen

Phosphorylated Human Stat3 Peptide

Reactivity

QC Testing : HumanTested in Development : Mouse

Applications

Intracellular staining (flow cytometry)

- Routinely Tested