货号：561038

品牌：BD Pharmingen

规格：1EA

目录价：￥1605.00

市场价格：￥1011.15

会员价格：￥882.75

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Catalog Number

561038

Brand

BD Pharmingen™

Name

CD25

Alias

IL-2 Receptor α chain, p55

Concentration

0.2 mg/ml

Size

25µg

RegulatoryStatus

RUO

Clone

PC61

Format

APC-Cy™ 7

Isotype

IgG₁, λ

Immunogen

IL-2-dependent cytolytic mouse T-cell clone B6.1

Strain Host

OFA

Reactivity

QC Testing : Mouse

Applications

Flow cytometry

- Routinely Tested

Clone - Description

PC61 reacts with the 55 kDa low affinity (α chain, p55, CD25) IL-2 receptor subunit which is expressed by activated T and B lymphocytes from all mouse strains tested. The IL-2 receptor α chain (IL-2Rα) by itself is not a signaling receptor. However, the IL-2 receptor α chain can combine with IL-2 receptor β chains and γ_c chains to form high affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express CD25. CD25 is transiently expressed at a low level during normal B-cell development at the Pre-B or Pre-B-II stage in the bone marrow, and it is expressed at a higher level during a very early stage of T cell development in fetal and adult thymus. The PC61 antibody recognizes an epitope of CD25 and appears to block binding of IL-2 to CD25, presumably by inducing a conformational change in CD25.^1,7

Clone - Application

Flow Cytometry: All formats of PC61 are useful for immunofluorescent staining and flow cytometry. Neutralization: Purified NA/LE™ PC61 is useful for neutralization.