货号：10602HNAE5

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：5 UG

目录价：￥637.00

市场价格：￥573.30

会员价格：￥573.30

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Manufactured by Sino Biological (China); Distributed by Life Technologies. Tumor necrosis factor alpha (TNF-α) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.This recombinant protein is expressed from a DNA sequence encoding the soluble form of human TNF (NP_000585.2) (Val 77-Leu 233) retaining the initial Met at the N-terminus.N-terminal Sequence Analysis: Met.Activity: measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EC₅₀ for this effect is typically 25-200 pg/mL, corresponding to a specific activity of 5 x 10⁶ to 4 x 10⁷ units/ mg.Formulation: lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80.Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.Expiration Date: expires one year from date of receipt when stored as instructed.BackgroundTumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF-α is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-α is primarily produced as a type I I transmembrane protein arranged in stable homotrimers, and a soluble trimeric form of TNF-α is released via proteolytic cleavage by the metalloprotease TACE/ ADAM17. Two receptors mediating the TNF-α function have been identified, the ubiquitous TNF RI from which most signaling are derived, and the hematopoietic cell-restricted TNF RII. Upon the TNF-α trimers binding, TNF receptors also form trimers, undergo a conformational change, and enable the adaptor protein TRADD to bind to the death domain, and thus initiates the signal pathways such as the NF-kB, Jak/STAT, and the MAPK pathways, as well as the death signaling. As a major mediator of apoptosis, inflammation and immunity, TNF-α has been implicated in the pathogenesis of a variety of human diseases including autoimmune diseases, insulin resistance, and cancer.You may also be interested in the following TNF-α products: ELISA KitsPrimary AntibodiesPathway Analysis Kits 规格

Form:	Lyophilized
Purity:	≥ 95% by SDS-PAGE
Species:	Human
Expression:	E. coli
Gene Symbol:	TNF
Gene Aliases:	TNF, DADB-70P7.1, DIF, TNF-alpha, TNFA, TNFSF2
Product Size:	5 µg
Protein Form:	Full Length,Recombinant
Protein Family:	Cytokines & Receptors
Protein Subtype:	TNF (Tumor Necrosis Factor)
Molecular Weight:	17.4 kDa
Research Category:	Immunology,Inflammation,Oncology,Signal Transduction
Protein Tag or Fusion:	Untagged
Validated Application:	Assay Standard

内容及储存 Includes 1 vial of lyophilized protein. Store at -20 °C.