货号：10928034

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：PLATINUM TAQ (25 RXNS)

目录价：￥3387.00

市场价格：￥3048.30

会员价格：￥3048.30

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR. Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific primers and target RNAs from either total RNA or mRNA. Reverse transcription automatically follows PCR cycling without additional steps.Using the SuperScript® One-Step SystemThe system consists of two major components: RT/ Platinum® Taq Mix and 2X Reaction Mix. The RT/ Platinum® Taq Mix contains a mixture of SuperScript® II Reverse Transcriptase and Platinum® Taq DNA Polymerase for optimal cDNA synthesis and PCR amplification. SuperScript® II RT is a modified version of Moloney Murine Leukemia Virus (M-MLV) RT, engineered to reduce RNase H activity and increase thermal stability. Platinum® Taq DNA polymerase is Taq DNA polymerase complexed with a proprietary antibody that inhibits polymerase activity at ambient temperatures. The antibody is denatured and polymerase activity is restored during the denaturation step in PCR cycling at 94°C. This provides an automatic "hot start" in PCR, increasing sensitivity, specificity, and yield. The 2X Reaction Mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg²⁺ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers. This convenient 2X format allows further addition of template and primer at any desired concentration. 规格

PCR Method:	1-Step RT-PCR
Reverse Transcriptase:	SuperScript® II
Polymerase:	Platinum® Taq DNA Polymerase
Hot Start:	Built-In Hot Start
Sample Type (General):	RNA
Product Size:	25 reactions
Detection Method:	Gel Electrophoresis
Shipping Condition:	Wet Ice
Final Product Size(s):	3 kb or less
Primer-Probe Compatibility:	Unlabeled Oligos

内容及储存 SuperScript® One-Step RT-PCR System components are listed in Table 1. Store at -20°C. Guaranteed stable for 6 months when properly stored.