货号:11137D
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:5 ML
目录价:¥13954.00
市场价格:¥12558.60
会员价格:¥12558.60
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描述
Dynabeads® CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or PBMC samples. Dynabeads® CD15 can also be used to positively isolate CD15+ cells for molecular applications. Advantages of Dynabeads® CD15:• Efficient depletion of granulocytes (neutrophils and eosinophils)• Isolation of granulocytes directly from whole blood for molecular applications• No columns requiredAbout Dynabeads® CD15Dynabeads® CD15 are uniform, superparamagnetic polystyrene beads (4.5 µm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.Magnetic bead-;based separation offers easy handlingDynabeads® CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.Learn more about Dynabeads® products• Find Dynabeads® products for a whole range of applications.• Find magnets for Dynabeads® separations.
规格
Capacity: |
Processes ~2x10^9 cells total |
Cell Type: |
Granulocytes |
Product Size: |
5 mL |
Target Species: |
Human |
Isolation Method: |
Depletion, positive isolation for molecular applications |
Output Viability: |
>95% |
Sample Type (Specific): |
Blood,PBMC |
Starting Material (Cell #): |
1x10^7 PBMCs per isolation |
Shipping Condition: |
Room Temperature |
内容及储存
This Product Contains: 5 ml Dynabeads® CD15 coated with anti-CD15 monoclonal antibody.Storage Conditions: 2°C to 8°C. Do not freeze.
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© ThermoFisher /Invitrogen/ Gibco/ ABI代理11137D DYNABEADS CD15:Store:CR : Cold Room (2 to 8 C),产品报价联系微信jinshanbio
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