货号:11304011
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:100 REACTIONS 95514, 52045
目录价:¥1965.00
市场价格:¥1768.50
会员价格:¥1768.50
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描述
Platinum® Taq DNA Polymerase High Fidelity is ideal for amplification of DNA fragments where high yields and robust amplification are required. High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´-5´ exonuclease activity) enzyme Pyrococcus species GB-D polymerase (Table 1). PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology.
• Fidelity – Greater than six times higher fidelity than Taq DNA polymerase
• Amplicon Size – Amplification of fragments up to 15 kb (Figure 1)
• Convenience – Room temperature reaction assembly
• Applications – Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCRUnit Definition: One unit of Platinum® Taq DNA Polymerase High Fidelity is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
规格
Polymerase:
|
Platinum® Taq DNA Polymerase High Fidelity
|
---|
Hot Start:
|
Built-In Hot Start
|
---|
Fidelity (vs. Taq):
|
6 X
|
---|
GC-Rich PCR Performance:
|
Low
|
---|
Reaction Format:
|
Separate Components
|
---|
Product Overhang:
|
Mixed
|
---|
Reaction Speed:
|
Standard
|
---|
Final Product Size(s):
|
20 kb or less
|
---|
Product Size:
|
100 reactions
|
---|
Exonuclease Activity:
|
5' - 3',3' - 5'
|
---|
Shipping Condition:
|
Dry Ice
|
---|
内容及储存
Platinum®
Taq DNA Polymerase High Fidelity is supplied with a vial of 10X High Fidelity Buffer [600 mM Tris-SO
4 (pH 8.9), 180 mM (NH
4)
2SO
4], and a vial of 50 mM MgSO
4.
Platinum® PCR SuperMix High Fidelity is supplied in a 1.1X concentration and contains Platinum®
Taq DNA Polymerase High Fidelity, dNTPs, salts, and MgSO
4.
Store at -20°C. Guaranteed stable for 6 months when properly stored.
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