货号：11308D

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：20 ML

目录价：￥12804.00

市场价格：￥11523.60

会员价格：￥11523.60

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Use this kit to enrich viable human dendritic cells (DC's) from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes. Highly enriched DC's are bead and antibody-free, and can be used in any functional assay. The cells should be further sorted to obtain high purity of the specific DC sub-populations of choice.• Obtain high recovery (80-100%) of viable DC's of all sup-populations.• Substantial reduction in sorting time and volume compared to sorting only, thus saving money as well.Methodology:An antibody mix towards the non- dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched human DC's can be directly sorted into the sup-population of choice or used in any other application.Starting samples:PBMC 规格

Capacity:	Processes ~2x10^9 cells total
Cell Type:	Dendritic Cells
Product Size:	2 x 10 mL
Target Species:	Human
Isolation Method:	Negative Isolation
Output Viability:	>95%
Sample Type (Specific):	PBMC
Starting Material (Cell #):	1x10^7 PBMCs per isolation
Shipping Condition:	Room Temperature

内容及储存 The Kit Contains: 2x10 ml Depletion MyOne™ Streptavidin Dynabeads® and 4 ml Antibody Mix containing an optimized mixture of biotinylated monoclonal antibodies towards CD3, CD14, CD16, CD19, CD56 and Glycophorin A.Store at 2-8°C