货号:11349D
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:10 ML, PROCESSES 1X10E9
目录价:¥10615.00
市场价格:¥9553.50
会员价格:¥9553.50
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Use this kit to isolate pure and viable untouched NK cells from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human NK cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free NK cells are truly untouched, and in perfect shape for any functional assay and application.• High purity, recovery and viability of human untouched NK cells• Easy to use and to scale up• No columns requiredMethodology:An antibody mix towards the non-NK cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human NK cells can be directly analyzed in a flow cytometer and used in any application.Starting Sample:PBMC
规格
Form: |
Bead |
Capacity: |
Processes ~1x10^9 cells⁄kit |
Cell Type: |
NK Cells |
Product Size: |
1 kit |
Final Product: |
Cells |
Target Species: |
Human |
Isolation Method: |
Negative Isolation |
Output Viability: |
>99% |
Number of Reactions: |
20 Reactions |
Sample Type (Specific): |
MNC |
Starting Material (Cell #): |
~5x10^7 cells/test |
Shipping Condition: |
Room Temperature |
内容及储存
The Kit Contains: 10 ml Depletion MyOne™ SA Dynabeads® and 2 ml Antibody Mix with biotinylated monoclonal antibodies towards human CD3, CD14, CD36, HLA Class II, CD123 and CD235a (Glycophorin A).
Store at 2-8°C
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