货号:11351D
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:10 ML 1X10E9
目录价:¥10504.00
市场价格:¥9453.60
会员价格:¥9453.60
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.• High purity, recovery and viability of human untouched B cells• Easy to use and to scale up• No columns requiredAn antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.Starting Sample:PBMC
规格
Form: |
Bead |
Capacity: |
Processes ~1x10^9 cells⁄kit |
Cell Type: |
B Cells |
Product Size: |
1 kit |
Final Product: |
Cells |
Target Species: |
Human |
Isolation Method: |
Negative Isolation |
Output Viability: |
>97% |
Number of Reactions: |
20 Reactions |
Sample Type (Specific): |
MNC |
Starting Material (Cell #): |
~5x10^7 cells/test |
Shipping Condition: |
Room Temperature |
内容及储存
The Kit Contains: 10 ml Depletion MyOne™ SA Dynabeads® and 2 ml Antibody Mix with biotinylated monoclonal antibodies towards human CD2, CD14, CD16 (a and b), CD36, CD43 and CD235a (Glycophorin A).
Store at 2-8°C
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