货号:11453D
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:10 ML (5 X 2 ML)
目录价:¥22405.00
市场价格:¥20164.50
会员价格:¥20164.50
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Dynabeads® Mouse T-Activator CD3/CD28 are 4.5 µm superparamagnetic beads with anti-CD3 and anti-CD28 antibodies covalently bound to the bead surface. Dynabeads® Mouse T-Activator CD3/CD28 is designed for the activation and expansion of mouse T cells for research purposes.• Activation of T cells without the need for feeder cells• Activated cells that retain in vivo-like function• Maximal reproducibility without contamination by soluble antibodies or mitogens• Up to 1,000-fold T cell expansion in two weeksSimple method for T cell activation Dynabeads® Mouse T-Activator CD3/CD28 offers a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet.
Starting samples Start with single-cell suspensions from spleen or lymph nodes, mononuclear cells (MNCs), whole blood, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells. Learn more about all of our Dynabeads® cell isolation products.Downstream applications The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.Learn more about Dynabeads® products• Find Dynabeads® products for a whole range of applications.• Find magnets for Dynabeads® separations.
规格
Cell Type: |
T Cells (Whole Population) |
Clonality: |
Monoclonal |
Reactivity: |
Mouse |
Product Size: |
5 x 2 mL |
Final Product: |
Cells |
Target Species: |
Mouse |
Sample Type (General): |
Cells |
Sample Type (Specific): |
Spleen,Lymph Node,Cell Cultures |
Shipping Condition: |
Room Temperature |
内容及储存
5 x 2 ml Dynabeads® Mouse T-Activator CD3/CD28 is supplied as a suspension containing 4 × 10
7 Dynabeads®/ml in phosphate buffered saline (PBS), pH 7.4, w/0.1% bovine serum albumin (BSA). Store at: 2°C to 8°C.
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