货号：11497013

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：1.5ML

目录价：￥8014.00

市场价格：￥7212.60

会员价格：￥7212.60

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Gibco® Sf21 cells are commonly used to isolate and propagate recombinant baculoviral stocks and to produce recombinant proteins. Gibco® Sf21 cells are adapted to serum-free suspension culture in Gibco® Sf-900™ II SFM, which saves significant time and expense associated with the adaptation of cultures. Gibco® Sf21 cells (frozen in Gibco® Sf-900™ II SFM) feature:• Recombinant protein expression from a variety of expression systems• Good growth in adherent or suspension culture• Documented lineage from a low passage Master Cell Bank• Quality and performance testingRecombinant protein expression from a variety of expression systemsHigh levels of protein expression in Sf21 cells can be obtained using either the BaculoDirect™ Baculovirus Expression System, the Bac-to-Bac® Baculovirus Expression System, or the InsectDirect® Expression System.Good growth in adherent or suspension cultureEach vial containing 1 × 10⁷ cells can be thawed directly into suspension or adherent culture. Protocols for adherent and suspension growth in Gibco® Sf-900™ II SFM are available in the product manual. Cultures can easily be transferred between the two conditions for ease of workflow but we recommend the stock cultures are maintained in suspension.Documented lineage from a low passage Master Cell BankGibco® Sf21 cells (in Sf-900™ II SFM) were prepared as serum-free, suspension cultures from Sf21 cells that originated at the USDA Insect Pathology Laboratory. The original IPLBSF-21 (Sf21) cell line was derived from the pupal ovarian tissue of the fall army worm, Spodoptera frugiperda. The serum-free Master Cell Banks were prepared at passage 10.Quality and performance testingEach lot of Gibco® Sf21 cells is tested for cell growth and viability post-recovery from cryopreservation. In addition, the Master Seed Bank has been tested for contamination of bacteria, yeast, mycoplasma and virus and has been characterized by isozyme and karyotype analysis.Product UseFor Research Use Only. Not for any animal or human therapeutic or diagnostic use. Caution: Handle as potentially biohazardous material under at least Biosafety Level 2 containment. This product contains Dimethyl Sulfoxide (DMSO), a hazardous material. Review the Material Safety Data Sheet before handling. 规格

Species:	Spodoptera frugiperda,S. frugiperda
Cell Line:	Sf21
Product Size:	1.5 mL
Media Adaptation:	Sf-900 II SFM (Serum-Free Media)
Shipping Condition:	Dry Ice

内容及储存 Storage conditions: Liquid nitrogen (vapor phase) Shipping conditions: Frozen