货号：11708021

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：250 REACTIONS #95514, #52806

目录价：￥3853.00

市场价格：￥3467.70

会员价格：￥3467.70

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Platinum® Pfx DNA Polymerase is ideal for amplification of DNA fragments for high-fidelity PCR applications. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3´→5´ exonuclease) activity. Platinum® antibody technology provides a simple, automatic, hot-start method that improves PCR specificity (see figure). PCR_x Enhancer Solution is included for higher primer specificity, broader magnesium concentration allowances, broader annealing temperature parameters, and improved thermostability of Platinum® Pfx DNA Polymerase. The PCR_x Enhancer Solution also helps optimize PCR of problematic and/or GC-rich templates. Platinum® Pfx DNA Polymerase provides:• 26 times higher fidelity than Taq DNA polymerase• Amplification of fragments up to 12 kb• Room temperature reaction assemblyApplicationsAmplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR. Platinum® Pfx DNA Polymerase is especially effective when very high levels of fidelity are required.Unit definitionOne unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C. 规格

Polymerase:	Platinum® Pfx DNA Polymerase
Hot Start:	Built-In Hot Start
Fidelity (vs. Taq):	26 X
GC-Rich PCR Performance:	Medium
Reaction Format:	Separate Components
Product Overhang:	Blunt
Reaction Speed:	Standard
Final Product Size(s):	12 kb or less
Product Size:	250 reactions
Exonuclease Activity:	3' - 5'
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Platinum® Pfx DNA Polymerase (250 units total, at 2.5U/µL) is a supplied with a vial (2 × 1 mL) of 10X PCRx Enhancer solution, a vial (2 × 1 mL) of 10X Pfx amplification buffer, and a vial (1 mL) of 50 mM MgSO₄. Designed for 250 reactions (at 1 unit each in a 50 µL reaction volume). Store at -20°C.