货号：11731015

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：(100 RXN)

目录价：￥6448.00

市场价格：￥5803.20

会员价格：￥5803.20

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Platinum® Quantitative RT-PCR ThermoScript™ One-Step System (1) is ideal for real-time quantitation of RNA molecules from total or poly(A)⁺ RNA in a single step. ThermoScript™ RT, a cloned avian reverse transcriptase, has been engineered to have reduced RNase H activity and high thermal stability (up to 70°C) to copy RNA with secondary structure and improve specificity of priming. Specificity and amplification efficiency are further enhanced through the use of Platinum® Taq DNA polymerase (3). The system can be used to quantitate the amount of specific mRNA from small numbers of cells (Figure 1).The Platinum® Quantitative RT-PCR ThermoScript™ One-Step System:• Is optimized to provide maximal performance with fluorescent detection chemistries employed in real-time quantitative PCR• Provides reduced RNase H activity for greater first-strand cDNA yield• Takes place sequentially in a single tube, under conditions optimal for both ThermoScript™ RT and Platinum® Taq DNA polymerase• Reduces reaction-to-reaction variability for improved precision in quantitative RT-PCR• Detects as little as 10 molecules of RNA template• Allows precise quantification of mRNA(s) from 0.1 pg to 1 µg of total RNA 规格

PCR Method:	1-Step qRT-PCR
Detection Method:	Primer-Probe Detection
Reaction Speed:	Standard
Sample Type (General):	RNA
Product Size:	100 reactions
Shipping Condition:	Dry Ice

内容及储存 Platinum® Quantitative RT-PCR ThermoScript™ One-Step System contains ThermoScript™ RT/Platinum® Taq mix, and 2X ThermoScript™ reaction mix (a buffer containing 0.4 mM of each dNTP, 6 mM MgSO₄ ). A vial of 50 mM MgSO₄ is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.