货号：11753500

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：500X50 UL RXNS

目录价：￥24154.00

市场价格：￥21738.60

会员价格：￥21738.60

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.Features of the CellsDirect™ One-Step qRT-PCR Kit include:• Efficiency—offers significant time and cost savings for increased throughput• Convenience—enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase• Sensitivity—no separate RNA isolation step increases sensitivity by minimizing sample loss• Broad detection range—detect targets from samples ranging from a single cell to 10,000 cells• Versatility—compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)Streamline your workflowIn traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.Ideal for your qRT-PCR applicationCellsDirect™ technology is validated for gene expression studies involving limited samples. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (see figure). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan® or LUX™ fluorogenic primers).Note: This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit (11754-500) with ROX™ Reference Dye included in the 2X Reaction Mix is also available. 规格

PCR Method:	1-Step qRT-PCR
Detection Method:	Primer-Probe Detection
Reaction Speed:	Standard,Fast
Platform:	Cepheid SmartCycler,BioRad iQ5,Corbett RotorGene,7500 Fast System,Stratagene Mx4000,Stratagene Mx3005P,Stratagene Mx3000P,MJ Opticon,MJ Chromo4,BioRad MyiQ,BioRad iCycler iQ,7500 System
Sample Type (General):	Cells
Product Size:	500 reactions

内容及储存 • 10 ml Resuspension Buffer • 1 ml Lysis Enhancer • 2 × 1.25 ml DNase I, Amplification Grade (1 U/µl) • 800 µl 10X DNase I Buffer • 2 × 1 ml 25 mM EDTA • 500 µl SuperScript® III RT/Platinum® Taq Mix • 12.5 ml 2X Reaction Mix • 1 ml 50 mM MgSO₄ • 12.5 ml DEPC-treated water • 10 µl HeLa Total RNA (10 ng/µl) • 500 µl ROX™ Reference Dye (store at -20°C in the dark) The kit is shipped on dry ice and should be stored at -20°C