货号：12033015

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：0.5 ML (5 X 0.1 ML)

目录价：￥2829.00

市场价格：￥2546.10

会员价格：￥2546.10

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 ElectroMAX™ DH10B™ T1 Phage-Resistant electrocompetent E. coli cells offer transformation efficiencies that are among the highest achieved by any product offered by Life Technologies, and can exceed 1 x 10¹⁰ cfu/µg plasmid DNA in efficiency. They are ideal for applications requiring high efficiency transformation, including cDNA and gDNA library construction. • Maximizes yield of transformants from limited cloning products • Permits efficient cloning of methylated DNA • Resistant to T1 and T5 bacteriophages • Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal • Delivers high yield plasmid preparations for downstream applications Phage-Resistant Representative Libraries from a Single Transformation The tonA mutation in DH10B™ confers resistance to bacteriophages T1 and T5 (1), safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX™ DH10B™ T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA, and allow for efficient plasmid rescue from eukaryotic genomes (2). ElectroMAX™ DH10B™ T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for applications where there DNA is limiting. The strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B™ an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification. Genotype: F^- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ^- rpsL nupG tonA Find the Strain and Format That You Need DH10B™ cells are available in both electrocompetent and chemically competent formats. Our ElectroMAX DH10B™ Cells are also suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection. Our MegaX DH10B™ T1^R Electrocomp™ Cells have the highest transformation efficiency (>3 x 10¹⁰ cfu/µg) and also offer the benefit of T1 and T5 phage resistance. For Research Use Only. Not intended for animal or human diagnostic or therapeutic use. 规格