货号：12183555

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：50 PREPS

目录价：￥3745.00

市场价格：￥3370.50

会员价格：￥3370.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The TRIzol® Plus RNA Purification Kit provides a simple, reliable, and rapid method for isolating high-quality total RNA from a wide variety of samples, including animal and plant cells, tissue, bacteria, and yeast. The kit combines the strong lysis capability of TRIzol® Reagent, followed by a convenient and time-saving silica-cartridge purification protocol from the PureLink® RNA Mini Kit, to provide ultrapure total RNA typically within an hour, even from difficult samples such as fibrous or fatty tissues. Features of this kit include: • Combined TRIzol®-based lysis and PureLink™ spin column-based RNA isolation technologies • Superior lysis capability and convenient protocolTwo products in one The TRIzol® Plus RNA Purification System couples the lysis capabilities of TRIzol® Reagent with the 1000 µg column-;binding capacity of the PureLink™ RNA Mini Kit, resulting in high RNA integrity and yield. The TRIzol® Plus RNA Purification System includes protocols for a variety of sample types, including fibrous or fatty tissues and plant material. The combination of TRIzol® Reagent followed by silica-cartridge purification of RNA is the recommended RNA purification method in the gene expression industry. The TRIzol® Plus RNA Purification Kit provides the reagents and an optimized protocol to purify total RNA for gene expression studies using an industry recommended method.Simple lysis to column purification protocol TRIzol® Reagent sample lysis maintains the integrity of the RNA, while disrupting cells and dissolving cell components. TRIzol® Reagent also provides an immediate and highly effective inhibition of RNase activity during sample homogenization. Following lysis, RNA in the lysed sample is bound to the clear silica-based membrane in the PureLink™ RNA Mini Kit Spin Cartridge, washed to remove contaminants, and then eluted in RNase-Free water (Tris Buffer, pH 7.5 may also be used). 规格

Sample Type (General):	Bacteria,Yeast,Cells,Plant Samples,Tissue
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Downstream Application:	RT-PCR (Endpoint),cDNA Library Construction,Nuclease Protection Assays,Nucleic Acid Labeling,Microarray Analysis,Real-Time PCR,Northern Blotting
Scale:	Mini
Format:	Spin Column
System:	TRIzol
Quantity:	50
Product Size:	50 preps
Final Product:	Total RNA
Yield (up to):	1000 µg
Purification Time:	20 min
Number of Reactions:	50 Preps
Isolation Technology:	Organic Extraction,Silica Spin Column
Starting Material (Amount):	Up to 200 mg tissue,Up to 5 x 10^8 cells

内容及储存 Kit Components: • TRIzol® Reagent (100 ml) • Lysis Buffer (125 ml) • Wash Buffer I (50 ml) • Wash Buffer II (15 ml) • RNase-Free Water (15.5 ml) • Spin Cartridges with collection tubes (50 each) • Collection Tubes (50 each) • Recovery Tubes (50 each) Store all components at room temperature. Sufficient reagents are provided to perform 50 RNA isolations.