货号：12328019

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：TRANSCRIPTASE

目录价：￥4566.00

市场价格：￥4109.40

会员价格：￥4109.40

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 AMV Reverse Transcriptase, Cloned, is used to synthesize first-strand cDNA for amplification by PCR or for cloning subsequent to conversion to double-stranded cDNA. Cloned Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT) is highly purified from insect cells infected with baculovirus containing the pol gene of AMV. The resulting enzyme has a higher specific activity than does native AMV RT, providing enhanced cDNA yields and improved sensitivity. The enzyme, accompanying buffers, and protocols are optimized to synthesize first-strand cDNA for amplification by PCR, and for cloning double-stranded cDNA of up to 12 kb. AMV RT has endoribonuclease H activity. Features of this enzyme:• Flexibility—works with oligo(dT), random primers, or gene-specific primers• Thermostability—AMV is active at temperatures up to 55°C• Multiple applications—first-strand cDNA synthesis for RT-PCR, and sequencing single- and double-stranded DNA and RNASourcePurified from insect cells infected with recombinant baculovirus expressing AMV pol.Unit definitionOne unit of cloned AMV RT is the amount of enzyme required to incorporate 1 nmole of dexoyribonucleotide into acid-precipitable material in 10 minutes at 37°C using poly(A)oligo(dT)₂₅ as template-primer. 规格

Optimal Reaction Temperature:	Up to 55° C
Final Product Size(s):	12.3 kb or less
Reverse Transcriptase:	AMV (Recombinant)
Ribonuclease H Activity:	Yes
Format:	Tube(s)
Quantity:	750 units
Template:	ssRNA
Sensitivity:	Low
Product Size:	750 units
Concentration:	15 U&⁄µl
Enzyme Function:	RNA dependent DNA Polymerase
Shipping Condition:	Dry Ice
Number of Reactions:	50 Reactions

内容及储存 Contents: • Cloned AMV RT (50 µL at 15U/µL) • 1 vial of 5X cDNA synthesis buffer [250 mM Tris-acetate pH 8.4, 375 mM potassium acetate, 40 mM magnesium acetate, and stabilizers] • 1 vial of 100 mM DTT • 1 vial of 40 mM magnesium acetate • 1 vial of 40 mM sodium pyrophosphate Store at -20°C. Stable for 12 months when stored at -20°C. Stability extended by storing at -70°C.