货号：12339024

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：1000 RX

目录价：￥14883.00

市场价格：￥13394.70

会员价格：￥13394.70

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The AccuPrime™ Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases (see figure). Platinum® anti-Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR. This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR. AccuPrime™ Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C. Advantages of AccuPrime™ Taq DNA Polymerase:• Specificity—control mispriming at every cycle to get single-band PCR products• Ease of use—minimize reaction optimization and primer set redesign• Convenience—conveniently assemble reactions at room temperatureAccuPrime™ buffersThe 10X AccuPrime™ buffers contain thermostable AccuPrime™ protein, Mg⁺⁺, and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR. Two individual buffer systems (10X AccuPrime™ PCR Buffer I and II) are provided for amplification of specific types of templates. 10X AccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).ApplicationsAccuPrime™ Taq DNA Polymerase can be used for a wide variety of applications, including multiplex PCR, TOPO TA Cloning®, and allele-specific amplifications. 规格

Polymerase:	AccuPrime™ Taq DNA Polymerase
Hot Start:	Built-In Hot Start
Fidelity (vs. Taq):	2 X
GC-Rich PCR Performance:	Low
Reaction Format:	Separate Components
Product Overhang:	3'-A
Reaction Speed:	Standard
Final Product Size(s):	5 kb or less
Product Size:	1,000 reactions
Exonuclease Activity:	5' - 3'
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 AccuPrime™ Taq DNA Polymerase is provided in a 1000 reaction kit. Each kit includes:• AccuPrime™ Taq DNA Polymerase (500 µL, at 2U/µL)• 10X AccuPrime™ PCR Buffer I* (2 × 1.25 mL)• 10X AccuPrime™ PCR Buffer II* (2 × 1.25 mL)• 50 mM Magnesium Chloride (500 µL)*10XAccuPrime™ PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids. 10X AccuPrime™ PCR Buffer II is designed for genomic DNA (200 bp–4 kb).10X AccuPrime™ PCR Buffer contains 200 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl₂, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol.Store at -20°C.