货号：12346094

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：1000 RXNS

目录价：￥17545.00

市场价格：￥15790.50

会员价格：￥15790.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 AccuPrime™ Taq DNA Polymerase, High Fidelity, amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and a proofreading enzyme, and AccuPrime™ accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases. With AccuPrime™ Taq DNA Polymerase, High Fidelity, you'll get:• The highest specificity and yield for the most robust PCR amplification• 9-fold higher fidelity than Taq DNA polymerase alone• Mimimal optimization steps, even with non-optimized primer sets• Efficient amplification of targets over a broad size range up to 20 kbHow it worksHigh fidelity is achieved by a combination of Platinum® anti-Taq DNA polymerase antibodies that inhibit polymerase activity, providing an automatic "hot-start", and the proofreading (3´→5´ exonuclease activity) enzyme Pyrococcus species GB-D. The thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, preventing mispriming and enhancing PCR specificity and yield. 规格

Polymerase:	AccuPrime™ Taq DNA Polymerase High Fidelity
Hot Start:	Built-In Hot Start
Fidelity (vs. Taq):	9 X
GC-Rich PCR Performance:	Low
Reaction Format:	Separate Components
Product Overhang:	3'-A,Mixed
Reaction Speed:	Standard
Final Product Size(s):	20 kb or less
Product Size:	1,000 reactions
Exonuclease Activity:	5' - 3',3' - 5'
Shipping Condition:	Dry Ice

内容及储存 AccuPrime™ Taq DNA Polymerase High Fidelity (200 µL, at 5U/µL)) is supplied with: • 50 mM MgSO₄ (1 mL) • 10X AccuPrime™ PCR Buffer I (4 × 1.25 mL) • 10X AccuPrime™ PCR Buffer II (4 × 1.25 mL) Buffer I and II differ in their concentration of thermostable AccuPrime™ protein. Their components are: 600 mM Tris-SO₄ (pH 8.9), 180 mM (NH₄)₂SO₄, 20 mM MgSO₄, 2 mM dGTP, 2 mM dATP, 2 mM dTTP, 2 mM dCTP, thermostable AccuPrime™ protein, 10% glycerol. The supplied PCR Buffer is a 10X concentrate and should be diluted for use. Buffer I is optimized for plasmids, cDNA, and λ DNA. Buffer II is optimized for genomic DNA. Store at -30°C to -10°C.