货号：12532024

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：5000 REACTIONS

目录价：￥102406.00

市场价格：￥92165.40

会员价格：￥92165.40

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Platinum® PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. You need only add template and primers, reducing set-up time by half (see figure). With Platinum® PCR SuperMix, High Fidelity, you'll:• Achieve greater than six-times higher fidelity than Taq DNA polymerase• Amplify fragments up to 15 kb• Produce products that are mixed blunt and 3-A' ends; however, the majority of ends will have a 3-A' overhangs• Minimize PCR optimizationUsing Platinum® PCR SuperMix, High FidelityPlatinum® PCR SuperMix, High Fidelity, is for high-specificity, high-fidelity DNA amplification applications, such as cloning or mutagenesis. High fidelity is provided by a mixture of Taq DNA polymerase and the proofreading Pyrococcus species GB-D polymerase. High specificity is achieved by anti-Taq antibodies, allowing for automatic "hot-start" PCR. This hot-start capability increases specificity and yield and allows for room-temperature reaction assembly. 规格

Polymerase:	Platinum® Taq DNA Polymerase High Fidelity
Hot Start:	Built-In Hot Start
Fidelity (vs. Taq):	6 X
GC-Rich PCR Performance:	Low
Reaction Format:	SuperMix or Master Mix
Product Overhang:	Mixed
Reaction Speed:	Standard
Final Product Size(s):	15 kb or less
Product Size:	5,000 reactions
Exonuclease Activity:	5' - 3'
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Platinum® PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 5,000 amplification reactions of 50 ?l each are provided.Platinum® PCR SuperMix High Fidelity Components:22 U/ml complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum® Taq Antibody; 66 mM Tris-SO4 (pH 8.9); 19.8 mM (NH4)2SO4; 2.4 mM MgSO4; 220 ?M dNTPs; and stabilizers.5,000 rxns: 4 × 56.25 mlSore at -20°C in a non-frost-free freezer.