货号：12535037

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：20 REACTIONS

目录价：￥8045.00

市场价格：￥7240.50

会员价格：￥7240.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR Cloning System with Gateway® Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway® Technology offers:• Rapid, efficient, directional cloning of PCR products• Versatility to use any amplification enzyme• Cloning without the need for restriction endonucleases or ligase• Selection of entry clones with kanamycin or Zeocin™In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use. 规格

Vector Type:	pDONR
Cloning Method:	Gateway®
Product Size:	20 reactions

内容及储存 The PCR Cloning Kit with Gateway® Technology includes Gateway® BP Clonase® II enzyme mix and reaction buffer, Gateway® pDONR™221 or pDONR™/Zeo vector, M13 sequencing primers, PEG/Mg²⁺ solution, proteinase K, pEXP7-tet positive control, and One Shot® OmniMAX™2 Competent Cells. Store competent cells and enzyme mix at -80°C. Store all other components at -20°C.