货号:12537102
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:20 REACTIONS
目录价:¥32216.00
市场价格:¥28994.40
会员价格:¥28994.40
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
MultiSite Gateway® Pro Technology enables you to efficiently and conveniently assemble multiple DNA fragments in the desired order and orientation into a Gateway® Expression vector. Using specifically designed att sites for recombinational cloning, you can clone two, three, or four DNA fragments into any Gateway® Destination vector containing attR1 and attR2 sites. The resulting expression clone is ready for downstream expression and analysis applications. The new MultiSite Gateway® Pro Technology allows you to: • Clone multiple DNA fragments into one vector without using restriction enzymes or ligases • Take full advantage of the wide selection of Gateway® Destination vectors (available from Invitrogen) or create your own • Replace multiple plasmid transfections with a single vector carrying all DNA elements of interest MultiSite Gateway® Pro Kits are available for cloning two, three, or four DNA fragments and for flexible and customized configuration of DNA fragments into a Gateway® Destination vector. All of the kits include internal positive control entry clones for troubleshooting potential entry clone issues.
规格
Promoter: |
None (Promoterless) |
Vector Type: |
MultiSite Gateway Vectors |
Key Function: |
Multi-Gene Expression,Clone your own Promoter |
Product Size: |
20 reactions |
Cloning Method: |
Gateway® |
Number of Fragments: |
2 Fragments |
内容及储存
The MultiSite Gateway® Pro Kits include vectors and primers as indicated in Table 1, One Shot® Mach1™ T1
R Chemically Competent Cells, and LR Clonase® II Plus and BP Clonase® II enzymes. Store cells at -80°C. Store Clonase® enzymes at -20°C for up to 6 months, or at -80°C for long-term storage. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
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