货号：12562062

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：5 REACTION

目录价：￥12579.00

市场价格：￥11321.10

会员价格：￥11321.10

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway® Technology with baculovirus expression makes the BaculoDirect™ System the fastest and easiest method for production of recombinant baculovirus.How it WorksThe BaculoDirect™ Linear DNA (the baculovirus genome) is engineered to include attR sites for quick and efficient recombination with a Gateway® entry clone. The gene of interest is recombined from the entry clone into the BaculoDirect™ Linear DNA using a simple, one-hour LR reaction (Figure 1). The resulting reaction mix contains the recombinant baculovirus carrying the gene of interest and is used directly to transfect insect cells. The need for transforming bacteria and isolating a large bacmid or co-transfection of a transfer vector and linear baculovirus DNA into insect cells is eliminated. As a result, the hands-on time is greatly reduced. Purified baculovirus can be isolated in less than one week.Gateway® Linear DNAThe BaculoDirect™ Linear DNA is designed for rapid cloning with a Gateway® entry clone and subsequent expression in Sf9 or Sf21 insect cells (Figure 2). The Linear DNA features:Strong polyhedrin promoter for high-level expressionR sites for efficient recombination with any attL-flanked Gateway® entry vectorTK gene for negative selection using ganciclovirN-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect™ N-Term Linear DNA)TEV protease cleavage site for removal of the V5-His tag following purification (BaculoDirect™ N-Term Linear DNA)C-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect™ C-Term Linear DNA) 规格

Cleavage:	TEV Protease Recognition Site
Promoter:	Polyhedrin
Vector Type:	BaculoDirect Vectors
Product Size:	5 transfections
Cloning Method:	Gateway®
Protein Tag or Fusion:	His Tag (6x),V5 Epitope Tag

内容及储存 The BaculoDirect™ Transfection Kits include five 0.3 µg vials of BaculoDirect™ Linear DNA, 125 µl of Cellfectin® transfection reagent, and ganciclovir. Store the BaculoDirect™ Linear DNA, and Cellfectin® at +4°C. All components are guaranteed stable for 6 months if stored properly