货号：12578043

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：20 CLONING REACTIONS

目录价：￥11966.00

市场价格：￥10769.40

会员价格：￥10769.40

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The GeneBLAzer™ C-terminal Gateway® Fusion Kit contains the Gateway®-adapted destinationvector, pcDNA™6.2⁄cGeneBLAzer™-DEST, designed for use with the GeneBLAzer™ Technology. ThepcDNA™6.2⁄cGeneBLAzer™-DEST vector enables easy fusing of any gene to a mammalian-optimized b-lactamase gene, bla, and facilitates in vivo or in vitro detection of β-lactamase reporter activity in mammalian cells using aunique fluorescent substrate. Use of the GeneBLAzer™ Technology provides ahighly sensitive and accurate method to quantitate gene expression inmammalian cells. The vector contains the following elements:• Human cytomegalovirus immediate-early (CMV) promoter⁄enhancer forhigh-level expression in a wide range of mammalian cells• β-lactamase bla(M) reporter gene for C-terminal fusion to the gene of interest• Two recombination sites, attR1 and attR2, downstream of the CMV promoterfor recombinational cloning of the gene of interest from an entry clone• Chloramphenicol resistance gene located between the two attR sites forcounterselection• The ccdB gene located between the two attR sites for negative selection• The Herpes Simplex Virus thymidine kinase polyadenylation signal forproper termination and processing of the recombinant transcript• f1 intergenic region for production of single-strand DNA in F plasmidcontainingE. coli• SV40 early promoter and origin for expression of the Blasticidin resistancegene and stable propagation of the plasmid in mammalian hosts expressingthe SV40 large T antigen• Blasticidin resistance gene for selection of stable cell lines• The pUC origin for high copy replication and maintenance of the plasmid inE. coli• The ampicillin resistance gene for selection in E. coli 规格

Promoter:	CMV
Key Function:	Reporter Assays
Protein Tag or Fusion:	BLA (Beta-Lactamase)
Reporter:	BLA (Beta-Lactamase)
Delivery Method:	Transfection
Cloning Method:	Gateway®
Selection Agent (Eukaryotic):	Blasticidin
Vector Type:	GeneBLAzer Vectors
Product Size:	1 kit
Constitutive or Inducible System:	Constitutive

内容及储存 GeneBLAzer® C-terminal Gateway® Fusion Kit contains 6 µg of pcDNA™6.2⁄cGeneBLAzer™-DEST vector and 10 µg of an expression control. Store these vectors at -20°C. In vivo detection kit contains 50 µg of CCF2-AM substrate (enough for 375 96-well reactions). Substrate should be stored at -20°C, dessicated, and protected from light. All reagents are guaranteed stable 6 months when properly stored.