货号：15206014

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：300 UN

目录价：￥640.00

市场价格：￥576.00

会员价格：￥576.00

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 5´-GT(Py)↓(Pu)AC-3´ 3´-CA(Pu)↑(Py)TG-5´Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	10 U/ µL
Contents	Hinc II (Hind II) 10X Buffer M (1mL) 10X Loading Buffer (1mL)
Reaction Temperature	37°C
Reaction Mixture	Hinc II 1 µL 10X Buffer M 2 µL Substrate DNA < 1 µg Sterile water to 20 µL
Heat inactivation	Enzyme is inactivated by heating at 65°C for 20 minutes.
Star Activity	Unrelated site may be cut in the presence of high enzyme concentration, or glycerol concentration >5%, and low ionic strength.
Effect of DNA Methylation	Enzyme activity is not affected by CG methylase.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of DNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer M	100 mM Tris-HCl, pH 7.5 100 mM MgCl2 10 mM Dithiothreitol 500 mM NaCl
Source	Haemophilus influenzae Rc

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)
Relative Activity (%)	20	100	20	40	100

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Enzyme:	Hinc II
Product Size:	300 units
Concentration:	10 U⁄µl
Compatible Buffer:	10X Buffer M
Methylation Sensitivity:	Not CpG methylation-sensitive
Optimal Reaction Temperature:	37° C
Sensitive to Heat Inactivation:	Yes (Heat-Inactivatable)
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Storage Buffer10 mM Tris-HCl, pH 7.5400 mM KCl0.1 mM EDTA1 mM DTT0.01% BSA50% (v⁄v) glycerolLoading Buffer1% SDS50% glycerol0.05% Bromophenol blueStore enzyme and buffer at -20°C, and loading buffer at RT.