货号：15240801

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：300 UN

目录价：￥1001.00

市场价格：￥900.90

会员价格：￥900.90

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 5´- C↓(Py)CG(Pu)G-3´ 3´- G(Pu)GC(Py)↑C-5´Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	10 U/ µL
Contents	BmeT110 I 10X K (1 µL) 10X loading dye (1 µL)
Reaction Temperature	37°C
Reaction Mixture	BmeT110 I 1 µL 10X K Buffer 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation	Enzyme is inactivated by heating at 80°C for 20 minutes.
Star Activity	Unrelated site may be cut in the presence of high concentrations of DMSO.
Effect of DNA Methylation	Enzyme activity is sensitive to CG methylase but unaffected by dam and dcm methylase.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Reaction Buffer M	10X Buffer K 200 mM Tris-HCl, pH 8.5 100 mM MgCl2 10 mM Dithiothreitol 1,000 mM KCl
Source	Bacillus megaterium T110

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)
Relative Activity (%)	<20	<20	20	100	<20

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Product Size:	300 units
Concentration:	10 U⁄µl
Compatible Buffer:	10X Buffer K
Methylation Sensitivity:	Not dam methylation-sensitive,CpG methylation-sensitive,Not dcm methylation-sensitive
Optimal Reaction Temperature:	37° C
Sensitive to Heat Inactivation:	Yes (Heat-Inactivatable)
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Storage Buffer10 mM Tris-HCl, pH 7.5100 mM KCl0.1 mM EDTA1 mM DTT0.15% Triton® X-1000.01% BSA50% (v⁄v) GlycerolLoading Buffer1% SDS50% glycerol0.05% Bromophenol blueStore enzyme and buffer at -20°C, and loading buffer at RT.