货号：15241001

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UN

目录价：￥1875.00

市场价格：￥1687.50

会员价格：￥1687.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 5´-A↓CCGGT-3´ 3´-TGGCC↑A-5´Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	10 U/ µL
Contents	BshT I 10X (1 µL) 0.1%BSA 10X loading dye (1 µL)
Reaction Temperature	37°C
Reaction Mixture	BshT I 1 µL 10X H Buffer 2 µL 0.10% BSA 2 µg Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation	Enzyme is inactivated by heating at 80°C for 20 minutes.
Star Activity	Unrelated site may be cut in the presence of low salt, high glycerol concentrations (>5%), pH >8.0, or a large excess of the enzyme.
Effect of DNA Methylation	Enzyme activity is unaffected by dam and dcm methylase but affected by CG methylase.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in 1 hour at 37°C.
10X Reaction Buffer M	10X Buffer H 500 mM Tris-HCl, pH 7.5 10 mM Dithiothreitol (DTT) 100 mM MgCl2 1000 mM NaCl
Source	Bacillus sphaericus Jo 22-024

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)
Relative Activity (%)	<20	20	80*	50	50

*100% activity is obtained in the presence of BSA.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Product Size:	100 units
Concentration:	10 U⁄µl
Compatible Buffer:	10X Buffer H
Methylation Sensitivity:	Not dam methylation-sensitive,CpG methylation-sensitive,Not dcm methylation-sensitive
Optimal Reaction Temperature:	37° C
Sensitive to Heat Inactivation:	Yes (Heat-Inactivatable)
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Storage Buffer10 mM Tris-HCl, pH 7.5100 mM KCl1 mM EDTA1 mM Dithiothreitol (DTT)0.2 mg⁄mL BSA50% (v⁄v) glycerolLoading Buffer1% SDS50% glycerol0.05% Bromophenol blueStore enzyme and buffer at -20°C, and loading buffer at RT.