货号：15413016

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：200 UN

目录价：￥1520.00

市场价格：￥1368.00

会员价格：￥1368.00

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 5´-GCATG↓C-3´ 3´-C↑GTACG-5´Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	10 U/ µL
Contents	Sph I 10X Buffer H (1mL) 10X Loading Buffer (1mL)
Reaction Temperature	37°C
Reaction Mixture	Sph I 1 µL 10X Buffer H 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation	Enzyme inactivated by heating at 65°C for 20 minutes.
Star Activity	Unrelated site may be cut under conditions of low ionic strength, high enzyme concentration, or glycerol concentration >5%.
Effect of DNA Methylation	Enzyme activity is not affected by CG methylase.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer H	500 mM Tris-HCl, pH 7.5 100 mM MgCl2 10 mM Dithiothreitol 1,000 mM NaCl
Source	Streptomyces phaeochromogenes

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)
Relative Activity (%)	20*	40*	100	120	20*

* weak star activity is detected For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Enzyme:	Sph I
Product Size:	200 units
Concentration:	10 U⁄µl
Star Activity:	May exhibit star activity
Compatible Buffer:	10X Buffer H
Methylation Sensitivity:	Not dam methylation-sensitive,Not CpG methylation-sensitive,Not dcm methylation-sensitive
Optimal Reaction Temperature:	37° C
Sensitive to Heat Inactivation:	Yes (Heat-Inactivatable)
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Storage Buffer10 mM Tris-HCl, pH 7.5100 mM KCl0.1 mM EDTA1 mM Dithiothreitol (DTT)0.15% Triton® X-1000.01% BSA50% (v⁄v) glycerolLoading Buffer1% SDS50% glycerol0.05% Bromophenol blueStore enzyme and buffer at -20°C, and loading buffer at RT.