货号：18080200

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：25 REACTIONS

目录价：￥4555.00

市场价格：￥4099.50

会员价格：￥4099.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The SuperScript® III CellsDirect cDNA Synthesis Kit is optimized for synthesizing first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA. Lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in cloning and PCR. For real-time quantitative RT-PCR, see the note below. Advantages of the SuperScript® III CellsDirect cDNA Synthesis Kit include: • Compatible with a wide range of mammalian cell types grown under different treatment conditions • Single-tube format minimizes reagent loss, sample loss, and handling time • Total lysate volume is used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts • SuperScript® III Reverse Transcriptase, with reduced RNase H activity and higher thermal stability, produces high yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts • Generates high-quality cDNA for use in a variety of applications, including cloning and PCR• Simple protocol takes less than 2 hours How it worksIn traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material. Using the SuperScript® III CellsDirect cDNA Synthesis System, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I is added to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell (as measured by serial dilution). The use of SuperScript® III Reverse Transcriptase ensures high specificity and high yields of cDNA from small amounts of starting material—as little as 10 pg total RNA. After synthesis, the first-strand cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations. Note: For real-time quantitative RT-PCR (qRT-PCR) from cell lysate, we recommend the Cells-to-CT™ family of products for RNA extraction. These kits include reagents and protocols that have been specifically optimized for real-time qRT-PCR. 规格

Reverse Transcriptase:	SuperScript® III
Downstream Application:	RT-PCR (Endpoint),Real-Time PCR
Reaction Format:	Separate Components
Sample Type (General):	Cells
Product Size:	25 reactions
Optimal Reaction Temperature:	50° C

内容及储存 The SuperScript® III CellsDirect cDNA Synthesis Kit includes SuperScript® III RT, RNaseOUT™ Ribonuclease Inhibitor, Oligo(dT)20 primers, DNase I, DNase I buffer, EDTA, dNTP mix, 5X first-strand buffer, 0.1 M DTT, RNase H, control RNA and primers, resuspension buffer, and DNase/RNase-free water. Store all components at -20°C. Guaranteed stable for 6 months when properly stored.