货号：18080300

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 REACTIONS

目录价：￥14432.00

市场价格：￥12988.80

会员价格：￥12988.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The SuperScript® III CellsDirect cDNA Synthesis Kit is optimized for synthesizing first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA. Lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in cloning and PCR. For real-time quantitative RT-PCR, see the note below. The kit has the following advantages:• Compatible with a wide range of mammalian cell types grown under different treatment conditions• Single-tube format minimizes reagent loss, sample loss, and handling time• Total lysate volume is used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts• SuperScript® III Reverse Transcriptase, with reduced RNase H activity and higher thermal stability, produces high yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts• Generates high-quality cDNA for use in a variety of applications, including cloning and PCR• Simple protocol takes less than 2 hoursHow it worksIn traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material. Using the SuperScript® III CellsDirect cDNA Synthesis System, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss. DNase I is added to eliminate genomic DNA prior to first-strand synthesis. This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell (as measured by serial dilution). The use of SuperScript® III Reverse Transcriptase ensures high specificity and high yields of cDNA from small amounts of starting material—as little as 10 pg total RNA. After synthesis, the first-strand cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations.Note: For real-time quantitative RT-PCR (qRT-PCR) from cell lysate, we recommend the SuperScript® III Platinum® CellsDirect Two-Step qRT-PCR Kit (Cat. Nos. 11737-030 and 11737-038) or the SuperScript® III Platinum® CellsDirect Two-Step qRT-PCR Kit with SYBR® Green (Cat. Nos. 11738-060 and 11738-068). These kits include reagents and protocols that have been specifically optimized for real-time qRT-PCR. 规格

Reverse Transcriptase:	SuperScript® III
Downstream Application:	RT-PCR (Endpoint),Real-Time PCR
Reaction Format:	Separate Components
Sample Type (General):	Cells
Product Size:	100 reactions
Optimal Reaction Temperature:	50° C

内容及储存 The SuperScript® III CellsDirect cDNA Synthesis Kit includes: • Resuspension Buffer (1 ml ) • RNaseOUT™ Recombinant Ribonuclease Inhibitor (40 units/µl) 200µl • DNase I (1 U/µl) 500 µl • 10X DNase I Buffer 160 µl • 25 mM EDTA 120 µl • Oligo(dT)₂₀ (50 µM) 120 µl • 10 mM dNTP Mix 100 µl • SuperScript™ III RT (200 units/µl) 100 µl • 5X RT Buffer 600 µl • 0.1 M DTT 100 µl • E. coli RNase H (2 U/µl) 100 µl • HeLa Total RNA (10 ng/µl) 10 µl • Forward Control Primer (10 µM) 10 µl • Reverse Control Primer (10 µM) 10 µl • E. coli RNase H (2 U/µl) 100 µl • HeLa Total RNA (10 ng/µl) 10 µl Store all components at -20°C.