货号：18162016

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：250 UN

目录价：￥3657.00

市场价格：￥3291.30

会员价格：￥3291.30

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA.Application: Labeling DNA with either radiolabeled or biotinylated nucleotides.Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964.Performance and Quality Testing: Performance tested in nick translation reaction.Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template•primer.Unit Reaction Conditions: 50 mM potassium phosphate (pH 7.5), 6.7 mM MgCl₂ , 1 mM 2-mercaptoethanol, 80 µg/ml template•primer, 32 µM dTTP, 69 nM [³H]dTTP, and enzyme in 100 µl for 30 min. at 37°C. 规格

Hot Start:	No
Polymerase:	DNA Polymerase I
Product Size:	250 units
Exonuclease Activity:	5' - 3',3' - 5'
Shipping Condition:	Wet Ice

内容及储存 DNA Polymerase I/DNase I contains 0.5 units DNA Polymerase I and 0.4 milliunits DNase I per microliter. Store at -20°C.