货号：18263012

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：1 ML (5 X 0.2ML) #18263

目录价：￥1917.00

市场价格：￥1725.30

会员价格：￥1725.30

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 10⁸ cfu/µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Features of these cells:• Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal• Maintains plasmid DNA with minimum recombination events• Delivers high-yield plasmid preparations for downstream applications• M13mp cloning vectors may be propagated on a lawn of DH5α-FT™, DH5αF'™, DH5αF'IQ™, JM101, or JM107 for plaque formationIdeal for difficult cloning projectsLibrary Efficiency® DH5α™ Competent Cells are recommended for routine cloning of genes into large plasmids, and are suitable for difficult clone constructions, such as blunt-end ligations. Library Efficiency® DH5α™ Competent Cells have a mid-range transformation efficiency (>1 x 10⁸ cfu/µg control DNA per 100 µL reaction). The strain also has the Φ80lacZΔM15 genotype, allowing blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the recA1 mutation helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation makes DH10B™ cells an excellent choice for amplifying plasmid DNA for subsequent extraction and purification.Genotype: F^- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(r_k^-, m_k⁺) phoA supE44 thi-1 gyrA96 relA1 λ^- 规格

Competent Cell Type:	Chemically Competent
Species:	E. coli
Format:	Tube(s)
Transformation Efficiency:	> 1x10^8
Bacterial or Yeast Strain:	DH5α™
Product Size:	10 reactions
Blue-White Screening:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
Reduces Recombination:	Yes
Cloning Methylated DNA:	No
Improves Plasmid Quality:	Yes
Propagating ccdB Vectors:	Not for ccdB vector propagation
F' Episome (to make ssDNA):	Lacks F' Episome
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA):	No
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Shipping Condition:	Dry Ice

内容及储存 Contains: • Library Efficiency® DH5α™ Competent Cells: 5 vials, 200 µl each (total of 1 ml) • pUC19 DNA (10 pg/ul): 1 vial, 50 µl • S.O.C. medium: 2 bottles, 6 ml each Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4C or at Room Temperature.