货号:18297010
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:1ML #18297
目录价:¥1446.00
市场价格:¥1301.40
会员价格:¥1301.40
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描述
MAX Efficiency® DH10B™ Competent Cells are highly efficient E. coli, perfect for most applications. DH10B™ E. coli are available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High-efficiency transformation is seen with plasmids 150 kb in size. The versatile DH10B™ genotype has the following features:• lacZ for blue/white screening of recombinant clones• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries• A1 mutation to increase plasmid yield and quantity
规格
Competent Cell Type: |
Chemically Competent |
Species: |
E. coli |
Format: |
Tube(s) |
Transformation Efficiency: |
> 1x10^9 |
Bacterial or Yeast Strain: |
DH10B™ |
Product Size: |
10 reactions |
Blue-White Screening: |
Yes |
Cloning Unstable DNA: |
Not Suitable for cloning unstable DNA |
Reduces Recombination: |
Yes |
Cloning Methylated DNA: |
Yes |
Improves Plasmid Quality: |
Yes |
Propagating ccdB Vectors: |
Not for ccdB vector propagation |
F' Episome (to make ssDNA): |
Lacks F' Episome |
Preparing Unmethylated DNA: |
Not Suitable for preparing unmethylated DNA |
T1 Phage - Resistant (tonA): |
No |
High Throughput Compatibility: |
Not High Throughput-Compatible (Manual) |
Shipping Condition: |
Dry Ice |
内容及储存
Contains:
• MAX Efficiency® DH10B™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• S.O.C. medium: 2 bottles, 6 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
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