货号:30342052
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:5000RXNS
目录价:¥8411.00
市场价格:¥7569.90
会员价格:¥7569.90
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Tfi DNA Polymerase is a new non-Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq, Tfi has 5'→3' exonuclease and 5'→3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness (Figure 1, A and B). Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. Tfi can be considered for use as a component in a diagnostic application if qualified by the end user. With Tfi DNA Polymerase, you'll:Eliminate licensing fees and restrictions associated with Taq-based enzymes• Save money-Tfi is half the price of Taq DNA Polymerase• Achieve yield, specificity, and fidelity comparable to Taq DNA polymerase• No need to reoptimize - swap out Taq for Tfi• Tfi is also available in special pack sizes and for OEM customers needing PCR kit manufacturing flexibility.
规格
Polymerase: |
Tfi DNA Polymerase |
Hot Start: |
No |
Fidelity (vs. Taq): |
1 X |
GC-Rich PCR Performance: |
Low |
Reaction Format: |
Separate Components |
Product Overhang: |
3'-A |
Reaction Speed: |
Standard |
Final Product Size(s): |
4 kb or less |
Product Size: |
5,000 reactions |
Exonuclease Activity: |
5' - 3' |
Shipping Condition: |
Dry Ice |
内容及储存
Tfi DNA Polymerase is supplied with a vial of magnesium-free 5X Tfi PCR Buffer [250 mM TrisHCl (pH 8.4), 75 mM (NH4)2SO4, 25 mM KCl, proprietary stabilizers, 1 mM DTT], and a vial of 50 mM MgCl2. Store at -20°C. Guaranteed stable for 6 months when properly stored.
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