货号：38042024

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：500 UN

目录价：￥2984.00

市场价格：￥2685.60

会员价格：￥2685.60

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Topoisomerase I (DNA-relaxing enzyme) catalyzes the removal of superhelical turns from covalently closed DNA by a transient breakage and rejoining of phosphodiester bonds. Topoisomerase I is active in the presence of EDTA.Applications: Relaxing positively and negatively supercoiled DNA (1). Producing DNA topoisomers (2).Source: Purified from calf thymus.Performance and Quality Testing: Endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease, and phosphatase assays; conversion of super-coiled DNA to relaxed DNA.Unit Definition: One unit catalyzes the conversion of 0.5 µg of superhelical Φ X174 RF DNA to a relaxed state in 30 min. at 37°C.Unit Reaction Conditions: 50 mM Tris-HCl (pH 7.5), 50 mM KCl, 10 mM MgCl₂ , 0.1 mM EDTA, 0.5 mM DTT, 30 µg/ml/BSA, 0.5 µgΦ X174 RF DNA, and enzyme in 50 µl for 30 min. at 37°C. 规格

Enzyme:	Topoisomerase
Product Size:	500 units
Shipping Condition:	Wet Ice

内容及储存 Store in freezer (-5 to -30°C).