货号：402300

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UG

目录价：￥5402.00

市场价格：￥4861.80

会员价格：￥4861.80

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ZO-1 Antibody (40-2300) Immunofluorescent analysis of ZO-1 (green) in Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 30 minutes at room temperature. Cells were stained with or without ZO-1 rabbit polyclonal antibody (Product # 70-2300), at a concentration of 5ug/ml for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification. Details
Tested species reactivity	Dog,Human,Mouse,Rat
Published species reactivity	Dog,Rat,Human,Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide derived from the N-terminal region of human, dog, mouse, and rat ZO-1
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/ml
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage Conditions	-20°C

Tested Applications	Dilution*
Immunocytochemistry (ICC)	5-10µg/ml
Immunofluorescence (IF)	5-10µg/ml
Immunohistochemistry (IHC)	Assay Dependent

^*Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

Published Applications
Immunocytochemistry (ICC)	See 4 publications below
Immunohistochemistry (Paraffin) (IHC (P))	See 1 publications below
Western Blot (WB)	See 1 publications below
Immunohistochemistry (IHC)	See 2 publications below
Miscellaneous PubMed (MISC)	See 1 publications below
Immunofluorescence (IF)	See 2 publications below
Immunoprecipitation (IP)	See 1 publications below

Product Specific Information

40-2300 has been successfully used in Immunofluorescence and Immunohistochemistry.

Background/Target Information

Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane- associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.