货号:4312765
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:+ PROTOCOL
目录价:¥5183.20
市场价格:¥4664.88
会员价格:¥4664.88
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
The Applied Biosystems® GeneAmp® Gold RNA PCR Core Kit has been optimized to combine reverse transcription using MultiScribe™ Reverse Transcriptase, with highly sensitive and specific amplification using AmpliTaq Gold® DNA Polymerase.• A two-enzyme/one-buffer system can perform either a one- or two-step reaction chemistry, offering ease of use and flexibility• AmpliTaq Gold® DNA Polymerase automates the hot-start PCR process,ensuring high specificity, high sensitivity, and increased yield• Can be used for multiplexing, saving both time and reagentsOne-Step PCRAmpliTaqGold® DNA Polymerase introduces automatic hot-start to RNA PCR. This kit is specifically designed to perform one-step RNA PCR. It also has the capability to perform two-step RNA PCR better than other available kits.Improved PerformanceThe GeneAmp® Gold RNA PCR Core Kit allows expression of multiple targets in the same reaction (multiplexing). It also simplifies the use of random hexamers and oligonucleotides(T) from a long and complicated two-step reaction to one easy step. This kit can be used to produce cDNAs up to a length of 5 kb, and can generate products from as few as 500 copies of known starting material. The GeneAmp® Gold RNA PCR Core Kit contains all the necessary reagents for 100 RNA PCR amplifications. Also available is the GeneAmp® Gold RNA PCR Reagent Kit, which contains the core and a control kit containing control RNA and control primers.Note: See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostic procedures.
详细说明
常用规格
PCR Method: |
1-Step RT-PCR,
2-Step RT-PCR
|
Reverse Transcriptase: |
MultiScribe™ Reverse Transcriptase
|
Polymerase: |
AmpliTaq Gold® DNA Polymerase
|
Final Product Size(s): |
5 kb or less
|
Hot Start: |
Built-In Hot Start
|
Sample Type (General): |
RNA
|
Platform: |
GeneAmp® 2400,
GeneAmp® 9600,
GeneAmp® 9700
|
Product Size: |
1 Kit
|
Detection Method: |
Gel Electrophoresis
|
Regulatory Statement: |
For Research Use Only. Not for use in diagnostic procedures.
|
Primer-Probe Compatibility: |
Unlabeled Oligos
|
内容和储存
Sufficient for 100 RNA PCR amplifications, 50 µl each. Store at –20°C.
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