货号：433280

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100UG

目录价：￥3435.00

市场价格：￥3091.50

会员价格：￥3091.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Rabbit anti-AF-6 (N-term); Polyclonal antibody specific to Mouse. This antibody is validated in Western Blot, and Immunohistochemistry. AF-6, also known as afadin, is expressed in a variety of cell types. At least 5 isoforms of this protein are produced by alternative splicing and the 'canonical' sequence is designated as l-afadin. Chromosomal aberration of the MLLT4 gene (encoding AF-6) is associated with acute leukemias. AF-6 exists as a homodimer and interacts with various components of the cellular adhesion system, including actin cytoskeleton. Nectin-afadin complex is involved in the formation of cell-cell junctions, such as adherens junctions (AJ) and tight junctions (TJ). AF-6 connects nectins to the cadherin-catenin system at AJs and to the claudin-ZO protein system at TJs. Afadin knock-out mice show embryonic lethality and embryonic stem cells from these mice show increased apoptosis. Nectin-afadin complex is involved in PDGF-induced cell survival, at least through the PI3K-Akt signaling pathway. Proper accumulation of Par complex and activation of cdc42 and aPKC, as well as laminin and integrin alpha 6 and integrin beta 1 deposition were also inhibited by knockout of afadin. This indicates that afadin organizes the formation of cell-cell junctions in early embryonic development. AF-6 is also a key modulator of Wingless⁄Wnt, Ras-Mitogen Activated Protein Kinase (MAPK) and Notch signaling pathways cross-communication. This happens through physical interaction with Ras, Notch and Dishevelled, a key Wingless effector. AF-6 also controls integrin-mediated cell adhesion. This is done at least partially by regulating Rap1 activation through recruitment of both SPA-1 (a Rap1 GTPase-activating protein) and Rap1GTP. These results demonstrate that AF-6 is an important molecule mediating cell-cell adhesion but is also capable of modulating several key signaling pathways. 详细说明 常用规格

Reactivity:	Mouse
Validated Application:	Western Blot, Immunohistochemistry
Host:	Rabbit
SubClass (Isotype):	Multi
Clonality:	Polyclonal
Antibody Fragment:	Whole Antibody
Target Gene:	MLLT3
Target Molecule:	AF-5
Gene ID:	17356
Gene Symbol:	RP3-431P23.2, AF6, AFADIN, FLJ34371
Label or Dye:	Unconjugated
Purification:	Pure
Concentration:	0.25 mg⁄ml
Form:	Liquid
Product Size:	100 µg
Volume:	400 µl
Shipping Condition:	Wet Ice
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

内容和储存

Store at 2-8°C for up to one month. Store at —20°C for long-term storage.