货号:4362070
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:PROTOCOL
目录价:¥2316.00
市场价格:¥2084.40
会员价格:¥2084.40
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
The GeneAmp® Fast PCR Master Mix, with its sensitive, pre-mixed hot-start chemistries, reduces PCR reaction time to as little as 25 minutes when used with the Veriti® 96-Well Thermal Cycler. Features of this master mix:• Set up reactions quickly, using premixed reagents• Detect a single copy of a gene in as little as 10 ng of DNA• Get reproducible, specific, and sensitive resultsEasy to usePre-mixed, hot-start chemistries enable you to set up your experiments quickly. And by generating greater than 90% "A" overhangs—it makes cloning easier too.Detect a single copy in 10 ng of DNAUse the GeneAmp® Fast PCR Master Mix to detect a single copy of a gene in 10 ng of human genomic DNA or as little as 10 copies of an HIV target.Part of the Applied Biosystems® Fast PCR SystemThe Applied Biosystems® Fast PCR System, consisting of the premixed GeneAmp® Fast PCR Master Mix, specially designed microplates and tubes, and the Veriti® 96-Well Thermal Cycler (0.1 mL or 0.2 mL), considerably reduces PCR reaction times.
规格
Polymerase: |
AmpliTaq® DNA Polymerase |
Hot Start: |
Built-In Hot Start |
Fidelity (vs. Taq): |
1 X |
GC-Rich PCR Performance: |
Medium |
Reaction Format: |
SuperMix or Master Mix |
Product Overhang: |
3'-A |
Reaction Speed: |
Fast |
Final Product Size(s): |
2 kb or less |
Product Size: |
250 reactions |
Exonuclease Activity: |
5' - 3' |
内容及储存
The GeneAmp® Fast PCR Master Mix contains a hot start polymerase system that does not require an activation step and has been optimized to decrease the overall PCR amplification time. The Fast PCR Master Mix is provided at 2X concentration for easier preparation of PCR reactions requiring only the addition of primers and templates. The GeneAmp® Fast PCR Master Mix contains two tubes (1.25 mL each) sufficient for 125 20µL PCR reactions. The Fast PCR Master Mix can also be ordered without a protocol.
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