货号:4364344
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:EACH
目录价:¥7785.00
市场价格:¥7006.50
会员价格:¥7006.50
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Everything you need for SYBR® Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. • Premixed components stored at 2–8°C significantly reduce assay setup time • SYBR® Green I dye detects double-stranded DNA, so specific probes are not required • AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase • Proprietary buffer enhancements ensure performance and reliability Maximum Flexibility and ConvenienceApplied Biosystems® SYBR® Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan® probes are required. SYBR® Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold® DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR® Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
规格
Detection Method: |
SYBR |
Platform: |
7300 System,7700 System,7500 System,StepOnePlus™, Standard Mode,QuantStudio™,ViiA™ 7 System,StepOne™, Standard Mode,7900HT System,7000 System |
Reaction Speed: |
Standard |
PCR Method: |
qPCR |
Sample Type (General): |
DNA |
Polymerase: |
AmpliTaq Gold® DNA Polymerase |
Label or Dye: |
SYBR® Green |
Product Size: |
2 x 5 mL |
Passive Reference Dye: |
ROX (Pre-mixed) |
Sample Type (Specific): |
cDNA,DNA (Genomic) |
Primer-Probe Compatibility: |
Unlabeled Oligos |
内容及储存
The 2 × 5 mL vials contain a 2X mixture of SYBR Green 1 Dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components. Sufficient reagents provided for 400 reactions based on a 50 µl reaction volume.Store at 2–8°C.
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