货号：4371353

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：EACH

目录价：￥1432.00

市场价格：￥1288.80

会员价格：￥1288.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The Applied Biosystems® TaqMan® Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications. • Distinct clusters and high call rates for unambiguous allelic discrimination • Validated with TaqMan® SNP Genotyping Assays • Excellent pre- and post-PCR stability for high throughput setup and analysis May be used for a variety of genotyping applications, including: • Candidate gene studies • Drug-target validation • Disease association studies • Population genetics • Linkage mapping • Agricultural applications Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.Exceptional Performance from an Optimized Mix (2X) Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include: • AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup. • Optimized mix components provide excellent specificity for discrimination between alleles • Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments • Single thermal cycling condition for consistent results with TaqMan® AssaysValidated with TaqMan® Genotyping Assays and Instruments TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, and Custom TaqMan® SNP Genotyping Assays. In addition, the master mix is validated with the Applied Biosystems® thermal cyclers and real-time PCR systems.High-Throughput Setup and Analysis The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend. Reliable Discrimination with Challenging Targets Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets.Setting the Industry Standard for Quality Extensive analytical and functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay and one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for 'Certificate of Analysis').Number of Genotyping Reactions at a Given Reaction Size: 4371355, TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL) • 50 µL Reactions: 400 • 20 µL Reactions: 1000 • 10 µL Reactions: 2,000 • 5 µL Reactions: 4,000 4381656, TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL) • 50 µL Reactions: 800 • 20 µL Reactions: 2,000 • 10 µL Reactions: 4,000 • 5 µL Reactions: 8,000 4371357, TaqMan® Genotyping Master Mix, (1 x 50 mL) • 50 µL Reactions: 2000 • 20 µL Reactions: 5,000 • 10 µL Reactions: 10,000 • 5 µL Reactions: 20,000 4381657, TaqMan® Genotyping Master Mix, (2 x 50 mL) • 50 µL Reactions: 4000 • 20 µL Reactions: 10,000 • 10 µL Reactions: 20,000 • 5 µL Reactions: 40,000 Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures. 规格

Platform:	7300 System,StepOne™, Standard Mode,7500 System,7900HT System,7000 System,StepOnePlus™, Standard Mode
Technique:	CNV (Copy Number Variation),SNP Genotyping (Real-Time PCR-Based)
PCR Method:	qPCR
Polymerase:	AmpliTaq Gold® DNA Polymerase
Product Size:	1 x 1 mL
Reaction Speed:	Standard
Detection Method:	Primer-Probe Detection
Genotyping Target:	Copy Number Variants,SNPs (Known),Drug Metabolizing Enzymes,SNPs (Unknown or Numerous)
Passive Reference Dye:	ROX (Pre-mixed)
Sample Type (General):	DNA
Sample Type (Specific):	DNA (Genomic)
Purity or Quality Grade:	UP (Ultra Pure)
Primer-Probe Compatibility:	TaqMan®

内容及储存 Supplied at 2X concentration. The mix is optimized for SNP genotyping using TaqMan® probes and contains AmpliTaq Gold® DNA Polymerase UP (Ultra Pure), dNTPs without dUTP, Passive Reference 1 and optimized mix components. This pack contains one 1 mL tube, sufficient for 40 reactions at the 50 µL total volume. Storage: 2-8°C Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).