货号：4380204

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：EACH

目录价：￥6979.00

市场价格：￥6281.10

会员价格：￥6281.10

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 This Applied Biosystems® product is for the fast, easy, glass fiber filter–based isolation of RNA from blood using standard laboratory equipment in about one hour. Contains sufficient reagents for RNA purification from 50 samples.• Isolate high-quality RNA from blood, suitable for real-time PCR and microarray analysis• Obtain highly stable RNA virtually free of RNase activity, contaminating gDNA, or RT-PCR inhibitors• Easy workflow requires only standard laboratory centrifuges and ~1 hour of time• No separate RBC lysis or proteinase K treatment requiredGene expression profiling and analysis of whole blood is becoming an increasingly important life science tool. Accurately measuring the molecular profile of blood-based RNA transcripts is a critical need in areas of research such as disease association studies, biomarker discovery and validation, and monitoring of drug treatment regimes.Accessory ProductThe highly unstable nature of RNA transcripts ex vivo creates an even greater challenge for accurate expression analysis. Ongoing metabolic changes can drastically affect gene expression profiles in as little as one hour in standard blood collection tubes (see Figure). Use of the Applied Biosystems® Tempus™ Blood RNA Tubes (SKU #4342792) is therefore recommended. These tubes are designed for direct collection of 3 mL of blood into a plastic, evacuated blood collection tube containing RNA Stabilizing Reagent. The RNA, and therefore the expression profile, is stable in the collection tube for up to five days at room temperature and minimally 7 days at 4°C (see Figure).Performance with EaseThe ability of the Tempus™ system (Tempus™ Blood RNA Tubes and Tempus™ Spin RNA Isolation Kit) to stabilize RNA expression profiles has been tested over a range of storage conditions (see Figure). The combination provides stable RNA, high yields of purified RNA (observed yields of 6–25 µg from a full blood draw from normal, healthy donors), and high-quality RNA (A260/280 ratio greater than 1.9 and RIN greater than 7.0) suitable for use in downstream applications such as quantitative PCR, microarray profiling, and other RNA analysis methods. 规格

Sample Type (General):	Blood,Plant Samples,Cells
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Downstream Application:	RT-PCR (Endpoint),Real-Time PCR,Microarray Analysis,Northern Blotting
Product Size:	50 preps
Final Product:	Total RNA
Yield (up to):	25 µg
Purification Time:	60 min
Number of Reactions:	50 Preps
Isolation Technology:	Silica Spin Column
Starting Material (Amount):	Up to 3 ml of blood

内容及储存 RNA purification filters, 2-mL collection tubes, 1X PBS, RNA Purification Resuspension Solution, RNA Purification Wash Solution 1, RNA Purification Wash Solution 2, and Nucleic Acid Purification Elution Solution should all be stored at room temperature.