货号：4386995

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：KIT (100)

目录价：￥8906.00

市场价格：￥8015.40

会员价格：￥8015.40

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The Ambion® TaqMan® Cells-to-C_T Control Kit is designed for use with the TaqMan® Gene Expression Cells-to-C_T Kit. The Control Kit includes XenoRNA™ Control, a synthetic RNA transcript with a unique sequence that lacks homology to current annotated biological sequences, making it an ideal control for any experiment. The Control Kit also includes a TaqMan® Gene Expression Assay for the XenoRNA™ Control target, and a TaqMan® Gene Expression Assay for the highly expressed endogenous control gene β-actin.Features of the The Ambion® TaqMan® Cells-to-C_T Control Kit:• Confirms sufficient cell input • Monitors the presence of RT or real-time PCR inhibitors • Controls for sample normalizationMonitor Inhibition and Confirm Sufficient Cell Input The TaqMan® Cells-to-C_T™ Control Kit is used to assess inhibition and cell input in Cells-to-C_T reactions. For example, the XenoRNA™ Control confirms that samples can support RT-PCR and is an indicator of reaction inhibitors. By adding the XenoRNA™ Control to the Stop Solution during the last step of the cell lysis procedure, each sample is provided with a constant amount of control target and subsequently amplified by the XenoRNA™ TaqMan® Gene Expression Assay. Amplification with the β-actin Gene Expression Assay functions as an endogenous control for normalization, and ensures that the lysis reaction contains a sufficient number of cells—this is important for experiments with fewer than ~100 cells and monitors the effectiveness of cell lysis.Evaluate Compatibility with New Cell Lines The TaqMan® Cells-to-C_T™ Control Kit is ideal for assessing the compatibility of untested cell lines with the TaqMan® Gene Expression Cells-to-C_T Kit to determine the maximum number of cells that can be used per lysis reaction. 规格

Format:	Tube(s)
Includes:	Control Primer-Probe Set(s) & Template(s)
Gene Symbol:	ACTB
Target Gene:	β-actin,XenoRNA™ control
Label or Dye:	FAM™
Product Size:	100 reactions
Control Template:	XenoRNA™ control (synthetic RNA transcript)
Internal Probe Modification:	MGB (Minor Groove Binder) (3'),Non-Fluorescent Quencher (3'),FAM (5')
Shipping Condition:	Dry Ice

内容及储存 Xeno™ RNA Control, 20X Xeno™ RNA Control Taqman® Gene Expression Assay, and 20X ACTB (β-actin) TaqMan® Gene Expression Assay are all stored at -20°C. All reagents are guaranteed stable for 6 months when properly stored.