货号：4402956

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：(100 RXN)

目录价：￥14672.00

市场价格：￥13204.80

会员价格：￥13204.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Ambion® Fast SYBR® Green Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results. A breakthrough cell lysis and RNA stabilization technology eliminates the laborious and time-consuming RNA purification process completely. The kit includes sufficient reagents for 100 reactions. The Fast SYBR® Green Cells-to-C_T™ Kit contains AmpliTaq® Fast DNA Polymerase, UP, which allows instant hot start, thus minimizing non-specific product formation with fast real-time PCR.• Includes optimized reagents to work efficiently, right out of the box• Simple, effective Cells-to-C_T™ technology enables sample preparation at room temperature in only 7 minutes, including DNase treatment• Validated accuracy, exceptional reproducibility, and maximal sensitivity from 10 to 100,000 cells per sample; results equivalent to those from purified RNAExtraordinary ValueThe kit includes reagents for sample preparation integrated into a complete, optimized gene expression workflow including reverse transcription reagents and a high-performance PCR master mix with SYBR® Green.Extraordinary EaseThe protocol begins with a simple 7-minute sample preparation procedure. First, cells are washed in PBS, and then lysed for 5 minutes at room temperature; DNase treatment can be performed concurrently. The lysis reaction is stopped, and the sample is then ready for reverse transcription or storage at –20°C for up to 5 months. Because samples are processed directly in culture plates (96- or 384-well), sample handling and the potential for sample loss or transfer error are minimized, resulting in higher reproducibility. Following sample preparation, a portion of the cell lysate is added to an RT reaction followed by real-time PCR using the included master mix.Extraordinary PerformanceTo demonstrate the performance of the kit, replicate experiments were run using either traditional RNA purification and real-time PCR analysis, or the FastSYBR® Green Cells-to-C_T™ Kit. The kit shows equivalent performance to results obtained using purified RNA for analysis of 10 to 100,000 cells. In addition, the sensitivity, efficiency, and dynamic range were shown to be superior to competitor lysate kits. Samples prepared with the kit have been tested for compatibility on various real-time PCR platforms including those from Applied Biosystems®, Roche, and Bio-Rad.Accessory Product:The SYBR® Green Cells-to-C_T™ Control Kit (SKU# 4402959) contains a Xeno™ RNA control and primer set and is recommended to monitor the efficiency of cell lysis and amplification inhibition in Cells-to-C_T™ reactions. 规格

PCR Method:	2-Step qRT-PCR
Purity or Quality Grade:	UP (Ultra Pure)
Reverse Transcriptase:	M-MLV Reverse Transcriptase
Polymerase:	AmpliTaq® Fast DNA Polymerase
Detection Method:	SYBR
Primer-Probe Compatibility:	Unlabeled Oligos
Reaction Speed:	Fast
Platform:	7500 Fast System,StepOnePlus™, Fast Mode,StepOne™, Fast Mode,7900HT Fast System
Sample Type (General):	Cells
Passive Reference Dye:	ROX (Pre-mixed)
Hot Start:	Built-In Hot Start
Label or Dye:	SYBR® Green
Product Size:	100 reactions
Green Features:	Less hazardous,Fewer resources used,Less waste
Shipping Condition:	Dry Ice

内容及储存 Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR®RT Buffer, Fast SYBR®Green PCR Master Mix at 4°C. Store Nuclease–free Water at room temperature.