货号:4440045
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:WITH UNG 5X5ML
目录价:¥26903.00
市场价格:¥24212.70
会员价格:¥24212.70
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
TaqMan® Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). You can use TaqMan® Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.
This TaqMan® Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX™ Passive Reference
• Uracil-N glycosylase (UNG)
• Optimized buffer componentsNote: This TaqMan® Universal Master Mix II contains Uracil-N glycoslyase (UNG). See a complete listing of all TaqMan® Universal Master Mix II products, with and without UNG here.
TaqMan® Universal Master Mix II has been optimized for use with primers and TaqMan® probes that have been designed according to Life Technologies' guidelines. The master mix can be used with custom TaqMan® assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan® Gene Expression Assays
• TaqMan® MicroRNA Assays
• TaqMan® Drug Metabolism Genotyping Assays
• TaqMan® SNP Genotyping Assays
For RNA quantitation experiments, the TaqMan® Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction using cDNA synthesis kits available from Life Technologies.
规格
Detection Method: |
Primer-Probe Detection |
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Platform: |
7300 System,7500 System,StepOnePlus™, Standard Mode,ViiA™ 7 System,StepOne™, Standard Mode,7900HT System |
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Reaction Speed: |
Standard |
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PCR Method: |
qPCR |
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Sample Type (General): |
DNA |
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Polymerase: |
AmpliTaq Gold® DNA Polymerase |
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Product Size: |
5 x 5 mL |
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Passive Reference Dye: |
ROX (Pre-mixed) |
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Sample Type (Specific): |
cDNA,DNA (Genomic) |
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Purity or Quality Grade: |
UP (Ultra Pure) |
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Primer-Probe Compatibility: |
TaqMan® |
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内容及储存
Store at 4°CGuaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
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