货号：4460382

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：EACH

目录价：￥60121.60

市场价格：￥54109.44

会员价格：￥54109.44

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 This TrueScience™ RespiFinder® Pathogen and Viral Identification Panel is a multiplex PCR–based test that can detect and differentiate up to 14 RNA viruses, 1 DNA virus, and 4 bacterial pathogens in a single tube (Table 1). The assay utilizes a reverse transcription PCR (RT-PCR)-based probe ligation technique that is rapid, sensitive, and accurate. Test results can be obtained typically within 8 hours of nucleic acid isolation. Because they are CE IVD–marked, you can be confident that TrueScience™ RespiFinder® Panels are designed to support the demanding needs of clinical environments by delivering unsurpassed data quality scientists and clinicians expect.Advantages of TrueScience™ RespiFinder® Panels • Broad spectrum – detect presence of up to 15 viral and 4 bacterial pathogens with one panel • Rapid test – diagnostic result available typically within 8 hours of nucleic acid isolation • Clarity of results – differentiate up to 19 different pathogens, with a single patient report • Minimal hands-on time – multiplex assay may reduce pipetting and sample transfer steps Detect Multiple Pathogens – One Tube, One Patient Sample Acute respiratory tract infection (RTI) is prevalent in adults and children and is of significant concern in immunocompromised patients. Both viruses and bacteria can cause acute RTI, and the number of potential causative agents is large. To further complicate diagnosis, a single patient can carry more than one infection. Rapid molecular diagnostic tests have the potential to detect and differentiate many serious pathogens, helping to reduce prescriptions for broad spectrum antibiotics.Broad Spectrum Molecular DiagnosticsThe TrueScience™ RespiFinder® Pathogen and Viral Identification Panels clearly indicate the presence of up to 19 targets in a single reaction, from a single patient sample. The assay differentiates the presence of multiple pathogens in the patient sample, allowing physicians to reduce the time to treatment by accurately diagnosing a broad spectrum of viruses and bacteria.Simple WorkflowThe TrueScience™ RespiFinder® Panels have a simple workflow, which starts with the combined reverse transcription and PCR pre-amplification of isolated total nucleic acids (RNA and DNA). Subsequently, probe hybridization, ligation, and amplification are performed (Figure 1). The result is a powerful multiplex assay that provides data lab directors and clinicians can easily interpret.Provide Accurate DetectionAfter PCR amplification with the TrueScience™ RespiFinder® Panel, pathogen detection proceeds on an Applied Biosystems® Genetic Analyzer, including the multicapillary systems such as the 3500 Series and 3130 Series Genetic Analyzers. The state-of-the-art 3500 Series Genetic Analyzers integrate seamlessly into your work environment, providing ease of use without sacrificing reliability. Hands-on time is reduced by preformulated, load-and-run consumables, which help reduce the possibility of mixing and handling errors.Clear ResultsApplied Biosystems® GeneMapper® Software v4.1 completes fragment analysis with a simple and easy-to-interpret analysis package for reporting results (Figure 2). Pathogen identification is conveniently called out in the GeneMapper® Software report. In addition to reporting the presence of multiple pathogens, GeneMapper® Software can also streamline the creation of a full patient sample report, which can be easily printed or transferred electronically.CE-IVD marked and validated with external QCMD standards* The TrueScience™ RespiFinder® Pathogen and Viral Identification Panels meet the requirements of the In Vitro Diagnostic Medical Device Directive. Not for sale in the USA. Not cleared by SFDA in China.* CE-IVD label applies to the kit only, secondary interpretation software is not part of the claim. Intended Use StatementThe TrueScience™ RespiFinder® 19 Pathogen Identification Panel and TrueScience™ RespiFinder® 15 Viral Identification Panel are qualitative multiparameter tests intended to simultaneously detect and identify common respiratory pathogens from purified total nucleic acids. The input sample is total nucleic acids extracted and purified from nasopharyngeal swabs, nasal aspirates, sputum, and bronchoalveolar lavages (BAL) from patients suspected of respiratory tract infections. Preparation of clinical samples is a separate process from the scope of the panels; use suitable methods or products to handle specimens and extract and purify nucleic acids. The TrueScience™ RespiFinder® Identification Panels aid in the diagnosis of respiratory tract infection when used in conjunction with other clinical and laboratory findings. Negative results do not necessarily indicate absence of viral or bacterial respiratory tract infection; negative results should not be used as the sole basis for diagnosis, therapy, or other treatment decisions. Positive results do not exclude co-infection with other pathogens. The pathogen(s) detected may not be the definite cause of disease. Other laboratory testing and assessment of clinical presentation must be included in the final diagnosis. Performance characteristics were established with validated EQA panels from www.qcmd.org. The product is for use by laboratory professionals, and it is intended for use with certain instruments and data analysis software from Life Technologies.DisclaimerThe results obtained from these or any other diagnostic panels should be used and interpreted only in the context of the overall clinical picture. Applied Biosystems cannot accept responsibility for any clinical decisions that are made. Note: The TrueScience™ RespiFinder® Pathogen and Viral Identification Panels meet the requirements of the In Vitro Diagnostic Medical Device Directive. Not for sale in the USA. Not cleared by SFDA in China. 详细说明 常用规格

Target Organism:	Coronavirus spp., Parainfluenza Virus (PIV), C. pneumonia, Influenza A, L. pneumophila, B. pertussis, Rhinovirus, Adenovirus spp., Influenza A H5N1, Human Metapneumovirus (hMPV), M. pneumoniae, Respiratory Syncytial Virus (RSV), Influenza B
Strain or Genotype:	RSV: Types A and B, Chlamydophila pneumonia: All types, Influenza: Influenza A, Influenza A H5N1, and Influenza B, Bordetella pertussis: All types, Coronavirus: 229E, NL63, and OC43, Human Metapneumovirus: All types, Mycoplasma pneumonia: All types, Adenovirus: All types, Rhinovirus: All types, Legionella pneumophila: All types, Parainfluenza: Types 1, 2, 3, and 4
Validated Application:	Sequencing
Detection Method:	Capillary Electrophoresis
PCR Method:	Multiplex PCR
Platform:	Veriti® Thermal Cycler, 3500 Genetic Analyzer, GeneAmp® 9700
Sample Type (Specific):	Bronchoalveolar Lavage (BAL), Nasal Wash, Trans-Tracheal Aspiration (TTA), Sputum, Throat Swab, Nasopharyngeal aspirate, Nasal Swab
Species:	Human
Product Size:	50 reactions
Workflow Step:	PCR Amplification, Reverse Transcription, Capillary Electrophoresis
Analysis Software:	GeneMapper® Software v4.1
Shipping Condition:	Dry Ice
High Throughput Compatibility:	Multiplexing

内容和储存

This kit contains 5X RT-PCR Buffer, dNTP Mix, RT-PCR Enzyme Mix, Internal Amplification Control (IAC), Pre-Amplification Primer Mix, Hybridization Buffer, Probe Mix, TwoStep Buffer, Ligase Enzyme, Taq Polymerase, Dilution Buffer, and Reference Marker (FAM™). Store at -15 to -25°C.