货号：491014

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：ANTIBODY

目录价：￥5790.00

市场价格：￥5211.00

会员价格：￥5211.00

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Unconjugated polyclonal antibody (crude serum) specific to human H3K27me3. This antibody is validated for use in Chromatin Immunoprecipitation (ChIP), ELISA, dot blotting and Western blotting. Crude serum from a rabbit immunized with the region of the histone H3 containing the trimethylated lysine 27 (K27me3). Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called "histone code". Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases. Levels of H3K27 dimethylation are higher in silent genes then in active genes than suggesting that this histone modification is associated with transcriptional repression. 详细说明 常用规格

Reactivity:	Human
Validated Application:	Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, ELISA
Host:	Rabbit
SubClass (Isotype):	IgG
Clonality:	Polyclonal
Target Gene:	H3K27me3
Target Molecule:	Peptides
Gene ID:	8290
Gene Symbol:	HIST3H3
Accession Number:	NM_003493
Label or Dye:	Unconjugated
Target Modification State:	Methylated
Purification:	Crude
Color:	Unconjugated
Form:	Frozen
High Throughput Compatibility:	Manual Protocols
Neutralizing Antibody:	No
Product Size:	100 µl
cGMP Compliance:	non-cGMP Compliant
Shipping Condition:	Dry Ice
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

内容和储存

Store at -20°C⁄ -80°C. Avoid multiple freeze-thaw cycles. Guaranteed stable for 6 months when properly stored.