货号：61006

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：KIT

目录价：￥6852.00

市场价格：￥6166.80

会员价格：￥6166.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The Dynabeads® mRNA Purification Kit rapidly isolates the mRNA transcriptome in typically 15 minutes, delivering pure, intact mRNA. The kit is designed to specifically target, capture, and purify mRNA molecules from total RNA preparations. • Obtain pure mRNA in typically 15 minutes • Recover and enrich the transcriptome efficiently • Prepare mRNA that is suitable for nearly every downstream applicationPure, Rapid mRNA Isolation The Dynabeads® mRNA Purification Kit contains magnetic beads for the isolation of the mRNA transcriptome from any total eukaryotic RNA preparation (Figure 1). Dynabeads® magnetic beads are uniform in size and highly mobile in solution (Figure 2). This enables the bead capture surface to quickly and continuously interact with the entire total RNA sample during the mRNA capture phase. The ribosomal RNA and small RNA molecules (transfer RNA, microRNA, small nucleolar RNA, and small cytoplasmic RNA) do not bind to the beads and are discarded. Only polyadenylated RNA species (mRNA) are captured resulting in cleaner, more sensitive results (Figure 3). Within about 15 minutes, pure mRNA is isolated and ready for use in downstream applications.A Straightforward Enrichment Procedure The Dynabeads® mRNA Purification Kit uses a robust affinity purification principle for the enrichment of polyadenylated mRNA. Superparamagnetic Dynabeads®, coupled to oligo-(dT)₂₅, are first equilibrated with Binding Buffer, and then mixed with purified total RNA. The beads are then washed to remove contaminating RNA species, and then mRNA is eluted in as little as 5 µl of 10 mM Tris-HCl. The entire process is facilitated by the use of a neodymium magnet (purchased separately), which allows for the quick and efficient immobilization of the magnetic beads during buffer changes.RNA is suitable for all downstream molecular applications including: • Gene cloning • cDNA synthesis, cDNA library construction • RT-PCR, Quantitative RT-PCR • RPA (Ribonuclease Protection Assay) • Subtractive Hybridization • Dot/slot Hybridization • Primer extension For Research Use Only. Not for human or animal therapeutic or diagnostic use. 规格

Sample Type (General):	RNA
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Downstream Application:	RT-PCR (Endpoint),Nuclease Protection Assays,Northern Blotting,Cloning,Real-Time PCR,cDNA Library Construction
Product Size:	2 mL
Final Product:	mRNA
Isolation Technology:	Magnetic Bead
Sample Type (Specific):	Total RNA
Shipping Condition:	Room Temperature

内容及储存 2-8°C2 ml Beads + Buffers