货号：700129

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 UG

目录价：￥4692.00

市场价格：￥4222.80

会员价格：￥4222.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 This purified ABfinity™ recombinant rabbit monoclonal antibody recognizes Creb [pS133].ABfinity™ Creb [pS133] antibody advantages include:• Superior lot-to-lot consistency• Extremely high specificity and sensitivity• Recombinant rabbit monoclonal antibody• Validation in Western Blot and Flow CytometrySpecific for Creb [pS133]CREB (cAMP responsive element binding protein) is a 37kDa transcription factor that belongs to the leucine zipper family (Ref 1). It binds to the cyclic-AMP response element as a homodimer (Ref 2). Upon hormone-dependent stimulation of the cyclic-AMP pathway, phosphorylated CREB1 acts as an inducer of transcription. CREB is phosphorylated at Ser133 by various signaling pathways including Ca²⁺ and stress signaling (Ref 3, 4). CREB1 is known to play important role in memory formation (Ref 2, 5). Aberration in CREB-mediated gene expression has been linked with Alzheimer disease hippocampus (Ref 6).Exceptional Consistency Helps Save You Time and Money ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with high consistency between lots, minimizing the need to revalidate your assays for each lot.High Specificity Means More Reliable DataThe proprietary recombinant technology employed in the production of ABfinity™ antibodies permits an extremely high degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Life Technologies has validated this antibody in Western Blot and Flow Cytometry.High Sensitivity Lets You Detect Low Levels of Target ProteinABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.Recombinant Rabbit IgG—Use Like Any Other IgG AntibodyAs with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150kDa band under non-reducing conditions, and 50kDa and 25kDa bands under reducing conditions.For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.Related Links• Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.• See Life Technologies' full line of ABfinity™ recombinant monoclonal antibodies.References

1. Hoeffler, J.P., Meyer, T.E., Yun, Y., Jameson, J.L., and Habener, J.F. (1988). Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA. Science 242, 1430-1433.

2. Bartsch, D., Casadio, A., Karl, K.A., Serodio, P., and Kandel, E.R. (1998). CREB1 encodes a nuclear activator, a repressor, and a cytoplasmic modulator that form a regulatory unit critical for long-term facilitation. Cell 95, 211-223.

3. Tan, Y., Rouse, J., Zhang, A., Cariati, S., Cohen, P., and Comb, M.J. (1996). FGF and stress regulate CREB and ATF-1 via a pathway involving p38 MAP kinase and MAPKAP kinase-2. The EMBO journal 15, 4629-4642.

4. Xing, J., Kornhauser, J.M., Xia, Z., Thiele, E.A., and Greenberg, M.E. (1998). Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation. Molecular and Cellular Biology 18, 1946-1955.

5. Dash, P.K., Hochner, B., and Kandel, E.R. (1990). Injection of the cAMP-responsive element into the nucleus of Aplysia sensory neurons blocks long-term facilitation. Nature 345, 718-721.

6. Satoh, J, Tabunoki, H., and Arima, K. (2009). Molecular network analysis suggests aberrant CREB-mediated gene regulation in the Alzheimer disease hippocampus. Disease Markers 27, 239-252.

规格

Reactivity:	Human
Validated Application:	Flow Cytometry,Western Blot
Host:	Mouse
SubClass (Isotype):	IgG1
Clonality:	Monoclonal
Clone ID:	B16H16L26
Antibody Fragment:	Whole Antibody
Gene ID:	1385
Gene Symbol:	Creb
Label or Dye:	Unconjugated
Target Modification State:	Phospho
Purification:	Purified
Concentration:	0.5 mg⁄ml
Form:	Liquid
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Mass:	100 µg
Neutralizing Antibody:	No
Product Size:	100 μg
Protein Family:	Transcription Factors
Quantity:	1 each
Volume:	100 µl
Research Category:	Signal Transduction
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 2-8°C for up to 1 month, -20°C for long term storage. Avoid repeated freezing and thawing.